Hali Crane
|
 |
In particular, the presence of PEG resulted in an aciclovir increase of mean size and size distribution. The Acyclovir / Aciclovir-loaded PEG-coated PECA nanospheres sho a significant (p 1.5% (w/v) should be used during preparation. The patients received one of four regimes of Acyclovir / Aciclovir and prednisolone, Varicella zoster virus (VZV) IgG, IgM, and IgA responses were measured by commercial and in-house enzyme immunoassays (EIA) using serum samples taken at days 0, 7, antibiotics and 21 after entry into the study. Effect of Acyclovir / Aciclovir and prednisolone on the serological response in herpes zoster.The serological response of patients with acute herpes zoster was studied to determine whether a diagnosis could be made on a single serum sample, and whether this response was modified by treatment with antiviral and/or steroid therapy. In vivo ocular bioavailability was evaluated by instilling 50 microL of the Acyclovir / Aciclovir-loaded nanospheres only once in the conjunctival sac of rabbit eyes. The influence of the presence of nonionic surfactant as well as other substances [i.e., 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CyD) and poly(ethylene glycol) (PEG)], on formulation parameters and loading capacity was investigated. The optimal time to detect either class of antibody was approximately 1 week after the onset of the vesicular rash, when 85% of patients had one or both classes of acute phase antibody in their serum. In the currie of release in phosphate buffer, PEG-coated nanospheres sho a slower release. This finding is probably due to an improved ocular mucoadhesion of PEG-coated PECA nanospheres.. There was no evidence of cross reaction with EBV, CMV, or HSV antibodies. At various time intervals, aqueous humour Acyclovir / Aciclovir content was determined by high-performance liquid chromatography. In vitro drug release from nanospheres was determined in both phosphate buffer (pH 7.4) and plasma. Ocular tolerability and in vivo bioavailability of poly(ethylene glycol) (PEG)-coated polyethyl-2-cyanoacrylate nanosphere-encapsulated Acyclovir / Aciclovir.Acyclovir / Aciclovir-loaded polyethyl-2-cyanoacrylate (PECA) nanospheres were prepared by an emulsion polymerization process in the micellar phase and characterized. Analysis was carried out on data from 71 patients. The presence of HP-beta-CyD elicited an increase of nanosphere size and size distribution, but zeta potential was not influenced. Neither treatment with prednisolone nor the length of therapy with Acyclovir / Aciclovir affected significantly the VZV IgM or IgA responses.
|